Course Outline

Introduction to Fiji and ImageJ

  • Overview of Fiji and its applications in scientific research
  • Understanding ImageJ’s architecture and plugins
  • Installing and setting up Fiji for optimal performance

Basic Image Processing Techniques

  • Image acquisition and import formats
  • Adjusting contrast, brightness, and applying filters
  • Image stacking and projection methods

Advanced Image Analysis

  • Segmentation techniques: thresholding and edge detection
  • Object counting and particle analysis in histological samples
  • Measuring areas, perimeters, and other morphological features

Working with Biological Samples

  • Processing images of histological tissues, blood cells, and algae
  • Color deconvolution and analysis of stained tissues
  • Time-lapse analysis and tracking cell movement

Automation and Customization

  • Creating and running macros for repetitive tasks
  • Using Fiji plugins for specialized analyses
  • Basic scripting with ImageJ Macro Language (IJM)

Data Management and Reporting

  • Exporting results and generating reports
  • Annotating images and preparing data for publication
  • Best practices for reproducible image analysis

Summary and Next Steps

Requirements

  • Basic understanding of biological imaging concepts
  • Familiarity with microscopy and histological techniques
  • Basic computer literacy

Audience

  • Researchers in biotechnology and environmental toxicology
  • Laboratory technicians working with histological and cellular imaging
  • Scientists interested in quantitative image analysis
 14 Hours

Delivery Options

Private Group Training

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  • Pre-course call with your trainer
  • Customisation of the learning experience to achieve your goals -
    • Bespoke outlines
    • Practical hands-on exercises containing data / scenarios recognisable to the learners
  • Training scheduled on a date of your choice
  • Delivered online, onsite/classroom or hybrid by experts sharing real world experience

Private Group Prices RRP from €4560 online delivery, based on a group of 2 delegates, €1440 per additional delegate (excludes any certification / exam costs). We recommend a maximum group size of 12 for most learning events.

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