In Situ Observation of Reduction Behavior of Cytochrome c Adsorbed on Glass Surface by Slab Optical Waveguide Spectroscopy

Naoki MATSUDA; Hirotaka OKABE; Masaki FUJII; Masayoshi MATSUI; Yusuke AYATO; Akiko TAKATSU; Kenji KATO

doi:10.1587/transele.E94.C.170

Special Section on Recent Progress in Molecular and Organic Devices

In Situ Observation of Reduction Behavior of Cytochrome c Adsorbed on Glass Surface by Slab Optical Waveguide Spectroscopy

Naoki MATSUDA, Hirotaka OKABE, Masaki FUJII, Masayoshi MATSUI, Yusuke AYATO, Akiko TAKATSU, Kenji KATO

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Keywords: slab optical waveguide spectroscopy, cytochrome c, adsorption, reduction reaction, solid/liquid interface

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2011 Volume E94.C Issue 2 Pages 170-175

DOI https://doi.org/10.1587/transele.E94.C.170

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Abstract

In situ observation of the adsorption process and the states of cytochrome c on glass/solution interfaces, and the functionality of the reduction reaction of adsorbed cytochorome c were performed by using slab optical waveguide (SOWG) spectroscopy. The peak position of the absorption band of cytochorome c adsorbed on a bare glass surface was almost the same as that of that in solution. The cytochorome c adsorbed on glass/solution interface was reduced by sodium dithionite solution. The adsorbed cytochorome c was still maintained its functionality after immobilization.

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