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@@ -5,17 +5,12 @@ Single cell papers with code can not only facilitate the reproducibility of biom
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'Papers with code' here means that authors provide necessary codes to reproduce figures or results in their papers.
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(Last update: May 09, 2023)
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(Last update: July 13, 2023)
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### Tutorial
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- [[code | Python]](https://github.com/theislab/single-cell-tutorial) Luecken, M.D., and Theis, F.J. (2019). **Current best practices in single‐cell RNA‐seq analysis: a tutorial.** Mol. Syst. Biol. 15, e8746.
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### Benchmark study
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- [[code | R]](https://github.com/oxwang/fda_scRNA-seq) Chen, W. et al. [**A multicenter study benchmarking single-cell RNA sequencing technologies using reference samples.**](https://www.nature.com/articles/s41587-020-00748-9) Nat. Biotechnol. (2020) doi:10.1038/s41587-020-00748-9.
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### Cell Atlas
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- [[code | R]](https://github.com/Single-Cell-Genomics-Group-CNAG-CRG/Tumor-Immune-Cell-Atlas) Nieto, P., Marc Elosua-Bayes, M., Trincado, J.L., Marchese, D., Massoni-Badosa, R., Salvany, M., Henriques, A., Mereu, E., Moutinho, C., Ruiz, S., et al. (2020). **A Single-Cell Tumor Immune Atlas for Precision Oncology.** BioRxiv 2020.10.26.354829.
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- [[code | R]](https://github.com/krasnowlab/HLCA) Travaglini, K. J. et al. **A molecular cell atlas of the human lung from single-cell RNA sequencing.** Nature 587, 619–625 (2020).
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- [[code | Python and R]](https://github.com/haniffalab/HCA_skin) Reynolds, G. et al. [**Poised cell circuits in human skin are activated in disease.**](https://www.biorxiv.org/content/10.1101/2020.11.05.369363v1) bioRxiv 2020.11.05.369363 (2020) doi:10.1101/2020.11.05.369363.
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- [[code | Python]](https://github.com/czbiohub/tabula-sapiens) Consortium TTS, Quake SR. The Tabula Sapiens: a multiple organ single cell transcriptomic atlas of humans. bioRxiv 2021; 2021.07.19.452956
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- [[code | Python and R]](https://github.com/niklaslang/PCLS_perturbations) Lang, N. J., Gote-Schniering, J., Porras-Gonzalez, D., Yang, L., De Sadeleer, L. J., Jentzsch, R. C., Shitov, V. A., Zhou, S., Ansari, M., Agami, A., Mayr, C. H., Kashani, B. H., Chen, Y., Heumos, L., Pestoni, J. C., Geeraerts, E., Anquetil, V., Saniere, L., Wögrath, M., … Schiller, H. B. (2023). [**Ex vivo tissue perturbations coupled to single cell RNA-seq reveal multi-lineage cell circuit dynamics in human lung fibrogenesis.**](https://www.biorxiv.org/content/10.1101/2023.01.16.524219v1) BioRxiv, 2023.01.16.524219. https://doi.org/10.1101/2023.01.16.524219.
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### Experimental research
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- [[code | R]](https://github.com/tgen/banovichlab) Habermann A, Gutierrez A, Bui L, Yahn S, Winters N, Calvi C, et al. **Single-cell RNA sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis.** Sci Adv 2020;6:eaba1972. https://doi.org/10.1101/753806.
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- [[code | R]](https://github.com/marzamKI/neurogenic_astros) Zamboni, M., Llorens-Bobadilla, E., Magnusson, J.P., and Frisén, J. (2020). **A Widespread Neurogenic Potential of Neocortical Astrocytes Is Induced by Injury.** Cell Stem Cell 1–13.
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### scATAC-seq
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- [[code | Python]](https://github.com/linnarsson-lab/ipynb-lamanno2016) La Manno, G., Gyllborg, D., Codeluppi, S., Nishimura, K., Salto, C., Zeisel, A., Borm, L.E., Stott, S.R.W., Toledo, E.M., Villaescusa, J.C., et al. (2016). **Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells.** Cell 167, 566-580.e19.
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- [[code | R, Python]](https://github.com/yal054/snATACutils) Li, Y.E., Preissl, S., Hou, X. et al. An atlas of gene regulatory elements in adult mouse cerebrum. Nature 598, 129–136 (2021). https://doi.org/10.1038/s41586-021-03604-1
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- [[code | R]](https://github.com/klarman-cell-observatory/ivPerturbSeq) Jin, X. et al. **In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with autism risk genes.** Science 370, eaaz6063 (2020).
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### Spatial transcriptomics
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- [[code | R]](https://github.com/saezlab/visium_heart/tree/master/snRNA_seq) Kuppe, C. et al. **Spatial multi-omic map of human myocardial infarction.** bioRxiv 2020.12.08.411686 (2020) doi:10.1101/2020.12.08.411686.
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- [[code | Python and R]](https://github.com/Teichlab/HCA_Heart_ver2) Kanemaru, K., Cranley, J., Muraro, D., Miranda, A. M. A., Ho, S. Y., Wilbrey-Clark, A., Patrick Pett, J., Polanski, K., Richardson, L., Litvinukova, M., Kumasaka, N., Qin, Y., Jablonska, Z., Semprich, C. I., Mach, L., Dabrowska, M., Richoz, N., Bolt, L., Mamanova, L., … Teichmann, S. A. (2023). [**Spatially resolved multiomics of human cardiac niches.**](https://www.nature.com/articles/s41586-023-06311-1) Nature. https://doi.org/10.1038/s41586-023-06311-1
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### Nanopore
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- [[code | Perl, Shell and R]](https://github.com/ucagenomix/sicelore) Lebrigand, K., Magnone, V., Barbry, P. & Waldmann, R. [**High throughput error corrected Nanopore single cell transcriptome sequencing.**](https://www.nature.com/articles/s41467-020-17800-6) Nat. Commun. 11, 4025 (2020).
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### Experimental research
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### scATAC-seq
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- [[code | R]](https://github.com/tgen/banovichlab) Habermann A, Gutierrez A, Bui L, Yahn S, Winters N, Calvi C, et al. **Single-cell RNA sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis.** Sci Adv 2020;6:eaba1972. https://doi.org/10.1101/753806.
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- [[code | R, Python]](https://github.com/yal054/snATACutils) Li, Y.E., Preissl, S., Hou, X. et al. An atlas of gene regulatory elements in adult mouse cerebrum. Nature 598, 129–136 (2021). https://doi.org/10.1038/s41586-021-03604-1
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- [[code | R]](https://github.com/marzamKI/neurogenic_astros) Zamboni, M., Llorens-Bobadilla, E., Magnusson, J.P., and Frisén, J. (2020). **A Widespread Neurogenic Potential of Neocortical Astrocytes Is Induced by Injury.** Cell Stem Cell 1–13.
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- [[code | Python]](https://github.com/linnarsson-lab/ipynb-lamanno2016) La Manno, G., Gyllborg, D., Codeluppi, S., Nishimura, K., Salto, C., Zeisel, A., Borm, L.E., Stott, S.R.W., Toledo, E.M., Villaescusa, J.C., et al. (2016). **Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells.** Cell 167, 566-580.e19.
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### Spatial transcriptomics
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- [[code | R]](https://github.com/klarman-cell-observatory/ivPerturbSeq) Jin, X. et al. **In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with autism risk genes.** Science 370, eaaz6063 (2020).
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- [[code | R]](https://github.com/saezlab/visium_heart/tree/master/snRNA_seq) Kuppe, C. et al. **Spatial multi-omic map of human myocardial infarction.** bioRxiv 2020.12.08.411686 (2020) doi:10.1101/2020.12.08.411686.
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### Benchmark study
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- [[code | R]](https://github.com/oxwang/fda_scRNA-seq) Chen, W. et al. [**A multicenter study benchmarking single-cell RNA sequencing technologies using reference samples.**](https://www.nature.com/articles/s41587-020-00748-9) Nat. Biotechnol. (2020) doi:10.1038/s41587-020-00748-9.
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### Trajectory analysis
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- [[code | Python]](https://github.com/rajewsky-lab/planarian_lineages) Plass, M., Solana, J., Alexander Wolf, F., Ayoub, S., Misios, A., Glažar, P., Obermayer, B., Theis, F.J., Kocks, C., and Rajewsky, N. (2018). **Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics.** Science. 360.
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### Visualization
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- [[code]](https://github.com/faryabib/NatMethods_TooManyCells_analysis) Schwartz GW, Zhou Y, Petrovic J, Fasolino M, Xu L, Shaffer SM, et al. **TooManyCells identifies and visualizes relationships of single-cell clades.** Nat Methods 2020. https://doi.org/10.1038/s41592-020-0748-5.

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