Abstract
Erythrocytes (RBC) are the most common type of blood cell. These cells are responsible for the delivery of oxygen to body tissues. The abnormality in erythrocyte cell affects the physical properties of red cell. It may also decrease the life span of red blood cells which may lead to stroke, anemia and other fatal diseases. Until now, Manual techniques are in practiced for diagnosis of blood cell’s diseases. However, this traditional method is tedious, time consuming and subject to sampling error. The accuracy of manual method depends on the expertise of the expert, while the accuracy of automated analyzer depends on the segmentation of objects in microscopic image of blood cell. Despite numerous efforts made for accurate blood cells image segmentation and cell counting in the literature. Still accurate segmentation is difficult due to the complexity of overlapping objects and shapes in microscopic images of blood cells. In this paper we have proposed a novel method for the segmentation of blood cells. We have used wiener filter along with Curvelet transform for image enhancement and noise removal. The snake algorithm and Gram-Schmidt orthogonalization have applied for boundary detection and image segmentation, respectively.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Meijering, E.: Cell Segmentation: 50 Years Down the Road  29(5), 140–145 (2012)
Savkare, S.S., Narote, S.P.: Automatic System for Classification of Erythrocytes Infected with Malaria and Identification of Parasite’s Life Stage. Procedia Technol. 6, 405–410 (2012)
Sharif, J.M., Miswan, M.F., Ngadi, M.A., Hj, S., Mahadi, M.: Red Blood Cell Segmentation Using Masking and Watershed Algorithm: A Preliminary Study pp. 27–28 (February 2012)
Shirazi, S.H., Haq, N., Hayat, K., Naz, S.: Curvelet Based Offline Analysis of SEM Images. PLoS ONE 9(8), e103942 (2014)
Xiao, X., Li, P.: An unsupervised marker image generation method for watershed segmentation of multiespectral imagery. Geoscience Journal 8(3), 325–331 (2004)
Duncan, J., Ayache, N.: Medical Image Analysis: Progress over two decades and thechallenges ahead. IEEE Transactions on Pattern Analysis and Machine Intelligence, Instituteof Electrical and Electronics Engineers (IEEE) 22(1), 85–106 (2000)
Kumar, V., Abbas, A.K., Fausto, N., Aster, J.: Robbins andCotran Pathologic Basis of Disease. Saunders, Philadelphia, PA (2010)
Suradkar, P.T.: Detection of Malarial Parasite in Blood Using Image Processing. International Journal of Engineering and Innovative Technology (IJEIT) 2(10) (April 2013)
Karel, Z.: Contrast limited adaptive histogram equalization. Graphics Gems IV, 474–485, code: 479–484 (1994)
Khan, M.I., Acharya, B., Singh, B.K., Soni, J.: Content Based Image Retrieval Approaches for Detection of Malarial Parasite in Blood Images. International Journal of Biometrics and Bioinformatics (IJBB)Â 5(2) (2011)
Ross, N.E., Pritchard, C.J., Rubin, D.M.: Duse, Automated image processing method for the diagnosis and classification of malaria on thin blood smears. Medical & Biological Engineering & Computing 44, 427–436 (2006)
Tek, F.B., Dempster, K.: Parasite detection and identification for automated thin blood film malaria diagnosis. Computer Vision and Image Understanding 114, 21–32 (2010)
Ruberto, C.D., Dempster, A., Khan, S., Jarra, B.: Analysis of Infected Blood Cell Images using Morphological Operators. Image and Computer Vision 20 (2002)
Angulo, J., Flandrin, G.: Automated detection of working area of peripheral blood smears using mathematical morphology. Analytical Cellular Pathology 25, 37–49 (2003)
Trivedi, M., Bezedek, J.C.: Low-level segmentation of Zacrial images with fuzzy clustering. IEEE Trans. on System Man and Cybernetics 16(4), 589–598 (1986)
ChulKo, B., Gim, J.W., Nam, J.Y.: Automatic white blood cell segmentation using stepwise merging rules and gradient vector flow snake. Micron 42, 695–705 (2011)
Sabino, D.M.U., da Fontoura Costa, L., Gil Rizzatti, E., Antonio Zago, M.: A texture approach to leukocyte recognition. Real-Time Imaging 10, 205–216 (2004)
Foran, D., Meer, P., Comaniciu: Image guided decision support system for pathology, machine vision and applications. Machine Vision and Applications 11(4), 213–224 (2000)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer International Publishing Switzerland
About this paper
Cite this paper
Shirazi, S.H., Umar, A.I., Haq, N.U., Naz, S., Razzak, M.I. (2015). Accurate Microscopic Red Blood Cell Image Enhancement and Segmentation. In: Ortuño, F., Rojas, I. (eds) Bioinformatics and Biomedical Engineering. IWBBIO 2015. Lecture Notes in Computer Science(), vol 9043. Springer, Cham. https://doi.org/10.1007/978-3-319-16483-0_18
Download citation
DOI: https://doi.org/10.1007/978-3-319-16483-0_18
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-16482-3
Online ISBN: 978-3-319-16483-0
eBook Packages: Computer ScienceComputer Science (R0)